EDDA-workflow

* EDDA = electrically detected displacement assay

With EDDA*, multiplexed DNA analysis can be completed within app. 1 h (20 min. nucleic acid isolation & purification, 30 min. PCR amplification and 5 – 10 min. for microarray hybridisation and read-out). The chip, a microelectrode array, is applied directly to the PCR amplication tube. There is no need for any labelling or washing steps. Based on established manufacturing processes of the semiconductor industry, the chip can be produced as low cost mass product.

 

EDDA-application

Besides the development of tests for the medical diagnostics industry (e.g. DNA subtyping of Human Papilloma Virus, subtyping of mycobacterium tuberculosis etc.) we offer simple to use customized chip set-ups (chip & primer & protocol) for own semi-quantitative, real time like PCR product analysis.