EDDA-principle

 

Schematic representation of the EDDA-process: the electrodes are modified with hybrids of capture- and signalling probe. The signalling probes are complementary to the expected target amplicons generated during asymmetric PCR. The electrode distant end of the signalling probe serves as a docking station for targets. At the electrode proximal end the signalling probes carry covalently attached ferrocenium moieties depicted as red sphere that generate the recordable signal. Hybridisation of the matching target releases the signalling probes thus inducing a significant reduction of the signal.

 

Sources

P. Liepold et al., Electrically detected displacement assay (EDDA): a practical approach to nucleic acid testing in clinical or medical diagnosis. Analytical and Bioanalytical Chemistry, Volume 391, Number 5 / July 2008.