Antibody detection

 

Modified Epstein-Barr-Virus (EBV)-antigene (AG) is attached to the electrode surface by dithiolanchor groups (small yellow dots). The modified electrode is incubated and washed with serum including specific antibodies (AB) for EBV-Virus.  Subsequently the electrode is treated with Anti-Human-IgG covalently derivatized to alkaline phosphatase (IgG-AP). Anti-Human-IgG binds to the specific antibodys. By adding of electrochemically inactive para-aminophenylphosphate (pAPP), the substrate of AP, para-aminophenole (pAP) is formed, which can be oxidized and detected at the electrode.

 

Sources

S. Neugebauer et al., Characterization of Nanopore Electrode Structures as Basis for Amplified Electrochemical Assays. Electroanalysis 18, 1929-1936 (2006).